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超声技术在中草药成分提取中的应用


  草药中所含的成分相当复杂,不仅含有有效成分,还含有无效成分。要提高中草药的治疗效果,必须把其有效成分提取出来,提取是直接关系到成品中有效成分含量及其内在质量、监床疗效、经济效益。
  目前实际生产中提取中草药有效成分的常见方法有浸渍法(常温浸渍法、温浸法、煎煮法)、渗漉法、回流法等,提取时间长,提出率也低。随着现代科学技术的发展,超声技术已广泛地应用于工业、农业、医药卫生等领域。由于该方法所需设备简单、操作方便、提取时间短、提取率高、节能、节约药材、无需加热等优点,其在提取中草药成分方面的应用受到了越来越多的重视。本文综述超声技术在中草药成分提取中的应用以及提取过程中应注意的问题。
  1、超声技术提取中草药成分的理论依据:
  超声波是一种弹性机械振动滤,是听觉阈以外的振动,它产生强烈振动,高速度,强烈的空化效应,搅拌作用;因此能破坏植物药材的细胞,使溶媒能渗透到药材细胞中,从而加速药材中的有效成分溶解于溶媒中,以提高有效成分的提出率。同时以常规方法提出的有效成分作对照,考察超声提取出的有效成分的结构是否有改变。据文献报道,两种方法所得到的有效成分进行薄层层析、红外光谱和核磁共振光谱的对比分析,两者所得到的图谱一致,说明超声提取不会改变有效成分的结构,并且缩短了提取时间,提高了提出率,从而为中草药成分的提取提供了一种快速、高产的新方法。
  2、超声技术在中草药成分提取中的应用
  2.1提取皂甙类成分皂甙类成分常用加水煎煮法或有机溶剂浸泡进行提取,耗时长,提出率低,采用超声技术则可缩短提取时间,提高提出率。宋小妹等从长梗绞股蓝中提取其主要有效成分绞股蓝皂甙,以75%乙醇回流为对照,超声提取3次,每次80min,可明显提高绞股蓝皂甙的得率;邵静枸等从党参中提取党参皂甙,以常规的溶剂浸渍法为对照,党参细粉经超声处理40min后,党参皂甙的提出率高出常规法(甲醇冷渍48h)1倍多,时间缩短了98.6%,而且经超声提取的党参皂甙得到的粗品量是常规法的近2倍,纯度也高;有关专家从穿山龙根茎中提到主要有效成分薯蓣皂甙,以70%乙醇浸泡48h为对照,用20kHz的超声波提取30min,其提出率是对照且的12倍,并用1MHz的超声波提取30min,其提出率是对照组的1.34倍,此工艺可以节约药材23.4%。由此看出,超声提取皂甙类成分具有省时、节约药材、杂质少、提出率高等优点。
  2.2提取生物碱类成分从中草药中用常规提取生物碱一般提取时间长,收率低,而经超声处理后可以获得很好的效果。从黄柏中提取小檗碱,以饱和石灰水浸泡24h为对照,用20kHz的超声波提取30min,提出率比对照组高18.26%,且小檗碱的结构未发生改变;郭孝武等从黄连根茎中提取黄连素,实验证明超声法优于浸泡法,即用20kHz的超声波处理30min,其提出率高达8.12%。在我国1985年版药典中,曾用超声技术提取木鳖碱(士的宁)、马钱子碱这些毒性成分,并明确规定:在药物含量测定时,用超声波处理40min,相当于冷浸24h。由此看出,应用超声技术提取得生物碱类成分的工艺简便、省时、提出率高。
  2.3提取黄酮类成分黄酮类成分常用加水煎煮法,碱提酸沉法或乙醇、甲醇浸泡提取,费时、费工、提取率低,而超声提取则可提高提出率,缩短提取时间。从黄芩根茎中提取主要有效成分黄芩甙,以水为溶剂,仅超声提取10min就高于加水煎煮3h的提取率,并以20kHz超声波提取40min的黄芩甙的提出率高。从槐米中提取芦丁,超声提取40min,其提出率为22.53%,是目前大生产得率的1.7-2倍,经对比试验可知,节约药材30-40%,具有较高的经济效益。
  2.4提取蒽醌类成分蒽衍生物在植物体朵存在形式复杂,游离态与结合态经常共存于同一种中草药中,一般提取都采用乙醇或稀碱性水溶液提取,因长时间受热而破坏其中的有效成分,影响提出率。从大黄中提取大黄蒽醌类成分,与常规烈煮法相比,用超声法提取10min比煎煮法提取3h的蒽醌类成分高,同时以频率为20kHz的超声波提取的提出率高,在复方首乌口服液的提取工艺中,对含有大量的蒽醌类衍生物的何首乌、大黄、番泻叶采用超声提取,从而避免蒽醌甙类物质因久煎破坏有效成分失败。

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超声技术在中草药成分提取中的应用

草药中所含的成分相当复杂,不仅含有有效成分,还含有无效成分。要提高中草药的治疗效果,必须把其有效成分提取出来,提取是直接关系到成品中有效成分含量及其内在质量、监床疗效、经济效益。


中药提取设备特点

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现代制药装备行业生产模式已焕然一新,新型理念助推生产和研发!

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湿法混合制粒机的制粒成品会受哪些因素的影响?

湿法混合制粒机是一种混合制粒设备,可以用以药物成分的混合。在工艺上,湿法混合制粒机是在把混合、制粒两步工艺合在一起,既节约了时间又满足了GMP要求,减少了交叉污染,提高了效率,是一种多快好省的办法,深受用户欢迎。

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